Abstract: Studies on agro-pastoralist’s perception to climate change have reported inconclusive evidences. This paper analysed agro-pastoralists’ perception on climate change from the gender perspective in Mvomero district in Tanzania. The study adopted cross sectional research design and involved 135 agro-pastoralists. Quantitative data were collected using semi structured questionnaire. Descriptive and inferential analysis was computed. Pearson’s Chi-square, summated index scale and an independent sample T-test were computed to establish relationship between key study variables. The study found higher level of awareness about climate change. Climate change was attributed to frequency of unfavourable climatic events such low rainfall, high temperature and unpredictable on set of rainfall seasons. Gender differences on the level of awareness about climate change was found however the relationship was not statistically significant (P>0.05). The perceptions on climate change differed between men and women although the relationship was not statistically significant (P>0.05). It is recommended for the government and nongovernmental organization espousing for improving the livelihood of agro-pastoralists to improve their knowledge of climate change and how to mitigate its impacts. Gender sensitive approaches should be introduced when training and assisting rural households’ decision making towards adopting coping and mitigating strategies to positively impact on gender roles, relations and wellbeing.